Research on the influence of nutrition on the production of space and quality herba Hyssopus officinalis l. (hyssop) cultivated ecological conditions in A.R.D.S. Secuieni

Hyssopus officinalis L. (hyssop) has been known as a medicinal plant since ancient times. Grown for herba contains volatile oil (0.5 and 1.3 % in dry herba between 0.10 and 0.15 % in fresh herba ), flavonoids, ursolic and oleanolic acids, carbohydrates, hisopina, tannins, resins, gums, sugars and a substance with antiviral marubiina, which gives a bitter taste. Due to the volatile oil constituents, the plant has antiseptic expectorant action and is bitter tonic (because marubiinei) and slightly astringent (due to tannins). Climatic conditions during the experiment were good, the average temperature in the two years was 9,150 C, the difference from the annual average (8.7ºC) being 0.45ºC. The amount of rainfall was 488.5 mm by 59.4 mm less than the annual average (552.1mm). The average for the two years of experimentation average number of branches per plant ranged from 14.27 branches/plant variant sown at 50 cm between rows and incineration (Mt.) and 23 branches/plant variant sown at 70 cm between rows and incineration. The average length of inflorescence was higher (12.25 cm) in variant sown distance of 70 cm between rows and 20 cm between plants in the row. Regarding the production of dry herba it was influenced by both the distance between rows (average factor A) and the distance between plants in the row (average factor B). The average for the two years of experimentation, production highest (2510 kg/ha) was obtained in the variant sown at a distance of 70 cm between rows and 20 cm between plants in the row. An average production close of 2368 kg/ha was obtained in variant sown at a distance of 70 cm between rows and 30 cm between plants in the row. Highest production of essential oil (80.88 l/ha) was obtained in the variant sown at a distance of 70 cm between rows and 20 cm between plants in the row

<i>Gemella sanguinis</i> Infective Endocarditis—Challenging Management of an 8-Year-Old with Duchenne Dystrophy and Undiagnosed Congenital Heart Disease: A Case Report

Congenital heart disease (CHD) remains a predisposing cardiac condition for infective endocarditis (IE). Case report: We present the case of 8-year-old boy with no known pre-existing cardiac disease diagnosed with infective endocarditis (IE) with Gemella sanguinis. After admission, he underwent transthoracic echocardiography (TTE), which revealed the presence of Shone syndrome with a bicuspid valve, mitral parachute valve and severe aortic coarctation. He developed a paravalvular aortic abscess with severe aortic regurgitation and left ventricle (LV) systolic dysfunction for which he required a complex surgical intervention after six weeks of antibiotic treatment, consisting of Ross operation and coarctectomy, with a complicated postoperative course, cardiac arrest and ECMO support for five days. The evolution was slow and favorable, with no significant residual valvular lesions. However, persistent LV systolic dysfunction and increased muscle enzymes required further investigation to establish a genetic diagnosis of Duchenne disease. As Gemella is not considered a frequent pathogen of IE, no current guidelines refer specifically to it. Additionally, the predisposing cardiac condition of our patient is not currently classified as “high-risk” for IE; this is not considered an indication for IE prophylaxis in the current guidelines. Conclusion: This case illustrates the importance of accurate bacteriological diagnosis in infective endocarditis and poses concerns regarding the necessity of IE prophylaxis in “moderate risk” cardiac conditions such as congenital valvular heart disease, especially aortic valve malformations

Emerging Strategies to Combat β-Lactamase Producing ESKAPE Pathogens

Since the discovery of penicillin by Alexander Fleming in 1929 as a therapeutic agent against staphylococci, &beta;-lactam antibiotics (BLAs) remained the most successful antibiotic classes against the majority of bacterial strains, reaching a percentage of 65% of all medical prescriptions. Unfortunately, the emergence and diversification of &beta;-lactamases pose indefinite health issues, limiting the clinical effectiveness of all current BLAs. One solution is to develop &beta;-lactamase inhibitors (BLIs) capable of restoring the activity of &beta;-lactam drugs. In this review, we will briefly present the older and new BLAs classes, their mechanisms of action, and an update of the BLIs capable of restoring the activity of &beta;-lactam drugs against ESKAPE (Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) pathogens. Subsequently, we will discuss several promising alternative approaches such as bacteriophages, antimicrobial peptides, nanoparticles, CRISPR (clustered regularly interspaced short palindromic repeats) cas technology, or vaccination developed to limit antimicrobial resistance in this endless fight against Gram-negative pathogens

DataSheet1_Alternative mitigating solutions based on inorganic nanoparticles for the preservation of cultural heritage.PDF

Introduction: Biodeterioration is a big challenge for the preservation of cultural heritage objects and for the community’s safety, fostering the search for novel methods effective in removing microbial biofilms and subsequent biodeterioration. In this context, nanoparticles (NPs) are considered an interesting alternative, based on their unique physico-chemical and biological properties.Methods: The present study aimed to evaluate the antimicrobial efficiency of Ag, Au, Cu, and ZnO NPs against a significant number of filamentous fungi and bacterial strains isolated from wooden and stone cultural heritage objects from different Romanian regions, as well as from museum collections, with the final goal to establish their potential to develop novel preservation strategies, which have high efficiency and low ecotoxicity.Results: Six types of nanoparticles (NPs) based on Ag, Au, Cu, and ZnO were synthesized and characterized for their physico-chemical properties, ecotoxicity, and efficacy against 75 filamentous fungi and 17 bacterial strains isolated from wooden and stone cultural heritage objects (15th–19th century). The results showed that all synthetized NPs are homogeneous, demonstrating a good stabilizing coating, and have spherical or triangular shapes, with sizes between 9 and 25 nm. The highest antifungal efficiency has been recorded for Ag NPs, followed by Cu NPs and ZnO NPs, with the most susceptible strains being Aspergillus montevidensis, Penicillium commune, Penicillium corylophilum, Bacillus megaterium, and B. cereus. The Cu NPs and ZnO NPs decreased the capacity of microbial strains to adhere to the inert substratum. The influence of the tested NPs against enzyme/organic acid production varied depending on the NP types and by species.Conclusion: The obtained results are promising for the development of efficient and economical alternative solutions for heritage preservation, showing high antimicrobial activity against the prevalent fungal and bacterial strains involved in the biodeterioration of Romanian heritage objects.</p